STEAP4 Polyclonal antibody

• Featured Product
• KD/KO Validated

STEAP4 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

34

Reviews (1)

Cat no : 11944-1-AP

Synonyms

TNFAIP9, STEAP family member 4, STAMP2, Six-transmembrane epithelial antigen of prostate 4, Metalloreductase STEAP4

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Validation Data Gallery

• 

  WB analysis using 11944-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 11944-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11944-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11944-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 11944-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 11944-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11944-1-AP (STEAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of 3T3-L1 using 11944-1-AP

  IP result of anti-STEAP4 (IP:11944-1-AP, 4ug; Detection:11944-1-AP 1:1000) with 3T3-L1 cells lysate 1200 ug.

• 

  IP experiment of 3T3-L1 using 11944-1-AP

  IP result of anti-STEAP4 (IP:11944-1-AP, 4ug; Detection:11944-1-AP 1:500) with 3T3-L1 cells lysate 1360 ug.

• 

  IHC staining of human prostate cancer using 11944-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11944-1-AP (STEAP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, 3T3-L1 cells, rat liver tissue, human liver tissue, human brain tissue, RAW 264.7 cells
• Positive IP detected in: 3T3-L1 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 27 publications below
• IHC: See 18 publications below
• IF: See 2 publications below

Product Information

11944-1-AP targets STEAP4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STEAP4 fusion protein Ag2545
• Full Name: STEAP family member 4
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC020600
• Gene Symbol: STEAP4
• Gene ID (NCBI): 79689
• RRID: AB_2197868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STEAP4 (also called STAMP2), is a member of the STEAP family. Structurally, It is a 459 amino acid protein characterized by a molecular topology of six transmembrane domains. The cytoplasmic N-terminal domain of STEAP4 shows structural similarity with bacterial and archaeal FNO oxidoreductase and STEAP4 exhibits a strong iron reductase activity. Studies in mice and human suggest that STEAP4 maybe involved in adipocyte development and metabolism, and may contribute to the normal biology of the prostate cell, as well as prostate cancer progression.

Publications

Species	Application	Title
human	IHC	J Allergy Clin Immunol; Six-transmembrane epithelial antigens of the prostate comprise a novel inflammatory nexus in patients with pustular skin disorders.; Authors - Yun Liang
mouse,rat	WB,IHC	Bone Res; Dietary fat-associated osteoarthritic chondrocytes gain resistance to lipotoxicity through PKCK2/STAMP2/FSP27.; Authors - Sung Won Lee ; KD Validated
mouse,human,rat	IHC,WB	Basic Res Cardiol; The six-transmembrane protein Stamp2 ameliorates pulmonary vascular remodeling and pulmonary hypertension in mice.; Authors - Mehreen Batool ; KO Validated
human	WB,IHC	Nat Commun; Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis.; Authors - Yun Liao
mouse	IF	Proc Natl Acad Sci U S A; Quantitative proteomics identifies STEAP4 as a critical regulator of mitochondrial dysfunction linking inflammation and colon cancer.; Authors - Xiang Xue
human, mouse	WB, IHC	J Hepatol; PMID: 25646886; Authors - Hye Y Kim ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiang (Verified Customer) (11-08-2019)

Works pretty good.

• Applications: Western Blot, Immunofluorescence,

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