STEAP3 Polyclonal antibody

STEAP3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28478-1-AP

Synonyms

dudlin 2, Dudulin 2, hpHyde, hTSAP6, Metalloreductase STEAP3, pHyde, STEAP family member 3, STEAP3, STMP3, TSAP6

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Validation Data Gallery

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  WB analysis using 28478-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28478-1-AP (STEAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 28478-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 28478-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 28478-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28478-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 28478-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 28478-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using STEAP3 antibody (28478-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, PC-12 cells, C6 cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human appendicitis tissue, mouse liver tissue, human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28478-1-AP targets STEAP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STEAP3 fusion protein Ag29528
• Full Name: STEAP family member 3
• Calculated Molecular Weight: 488 aa, 55 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC042150
• Gene Symbol: STEAP3
• Gene ID (NCBI): 55240
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STEAP3 (Six-Transmembrane Epithelial Antigen of Prostate 3) is also named as TSAP6, Dudulin-2 and pHyde, and belongs to the STEAP family. STEAP3 is a member of the STEAP family and is composed of a six-transmembrane domain at the COOH-terminal domain and a cytoplasmic N-terminal oxidoreductase domain, which is essential for iron and copper uptake (PMID:16227996). STEAP3 contains a functional p53-binding site in its promoter and can be upregulated following p53 activation to enhance cell death in myeloid leukemia cell line and breast cancer cells (PMID: 18617898). By interacting with Nix, a pro-apoptotic Bcl-2 family member, and Myt1 kinase, a negative regulator of the G2/M transition, STEAP3 overexpression promotes apoptosis and inhibits G2/M transition in cell cycle progression (PMID: 12606722, PMID: 10504341).