Stanniocalcin 2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Stanniocalcin 2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 10314-1-AP

Synonyms

STC2, STC related protein, STC 2, Stanniocalcin-related protein, Stanniocalcin-2

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Validation Data Gallery

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  IHC staining of human renal cell carcinoma using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of U2OS using 10314-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Stanniocalcin 2 antibody (10314-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human renal cell carcinoma tissue, human lung cancer tissue, human colon cancer tissue, human kidney tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 10 publications below
• IF: See 2 publications below

Product Information

10314-1-AP targets Stanniocalcin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Stanniocalcin 2 fusion protein Ag0359
• Full Name: stanniocalcin 2
• Calculated Molecular Weight: 33 kDa
• GenBank Accession Number: BC000658
• Gene Symbol: Stanniocalcin 2/STC2
• Gene ID (NCBI): 8614
• RRID: AB_2197546
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.

Publications

Species	Application	Title
human	WB,IHC	Eur Urol; Identification of stanniocalcin 2 as prognostic marker in renal cell carcinoma.; Authors - Meyer Hellmuth-A HA
human	WB	Proc Natl Acad Sci U S A; Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.; Authors - Lei Shi
human	IHC	Genomics Proteomics Bioinformatics; miRNA-mRNA Regulatory Network and Factors Associated with Prediction of Prognosis in Hepatocellular Carcinoma.; Authors - Bo Hu
human	IHC	Int J Cancer; Additive effect of the AZGP1, PIP, S100A8, and UBE2C molecular biomarkers improves outcome prediction in breast carcinoma.; Authors - Parris Toshima Z TZ
human	IHC	Endocr Relat Cancer; Tumor suppressor functions of DAXX through histone H3.3/H3K9me3 pathway in pancreatic NETs.; Authors - Hiroki Ueda
human	WB	Sci Rep; Five hypoxia and immunity related genes as potential biomarkers for the prognosis of osteosarcoma.; Authors - Dachang Liu