STAT6 Monoclonal antibody, PBS Only

STAT6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E5B8

Cat no : 66717-1-PBS

Synonyms

D12S1644, IL 4 STAT, STAT6, STAT6B, STAT6C

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Validation Data Gallery

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  WB analysis using 66717-1-Ig (same clone as 66717-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66717-1-Ig (STAT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66717-1-Ig (same clone as 66717-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66717-1-Ig (STAT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66717-1-Ig (same clone as 66717-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66717-1-Ig (STAT6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66717-1-Ig (same clone as 66717-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66717-1-Ig (STAT6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66717-1-PBS targets STAT6 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: STAT6 fusion protein Ag0410
• Full Name: signal transducer and activator of transcription 6, interleukin-4 induced
• Calculated Molecular Weight: 100 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC005823
• Gene Symbol: STAT6
• Gene ID (NCBI): 6778
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.