STAT5B Monoclonal antibody, PBS Only

STAT5B Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D9B11

Cat no : 66427-1-PBS

Synonyms

STAT5A/B, STAT5, 1D9B11

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Validation Data Gallery

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  WB analysis of Jurkat using 66427-1-Ig (same clone as 66427-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66427-1-Ig (STAT5B Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66427-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66427-1-Ig (same clone as 66427-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66427-1-Ig (STAT5B Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66427-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66427-1-Ig (same clone as 66427-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STAT5B antibody (66427-1-Ig, Clone: 1D9B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66427-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66427-1-Ig (same clone as 66427-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT5B antibody (66427-1-Ig, Clone: 1D9B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66427-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 66427-1-Ig (same clone as 66427-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human STAT5B (66427-1-Ig, Clone:1D9B11) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG2a (KFA042), or 0.5 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66427-1-PBS in a different storage buffer formulation.

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Product Information

66427-1-PBS targets STAT5B in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: STAT5B fusion protein Ag2709
• Full Name: signal transducer and activator of transcription 5B
• Calculated Molecular Weight: 787 aa, 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC020868
• Gene Symbol: STAT5B
• Gene ID (NCBI): 6777
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

STAT5B belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B carries out a dual function: signal transduction and activation of transcription. STAT5B mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different GH. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. Despite the 95% homology between the immunogen (Ag2709) and the STAT5A protein, our WB detection showed that this antibody does not recognize the STAT5A fusion protein (Ag19546).