STAT3 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

STAT3 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G2D12

Cat no : 60199-1-PBS

Synonyms

Acute phase response factor, APRF, HIES, STAT3

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Validation Data Gallery

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  WB analysis of HeLa using 60199-1-Ig (same clone as 60199-1-PBS)

  WB result of STAT3 antibody (60199-1-Ig, 1:3000) with si-Control and si-STAT3 transfected HeLa cells. This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  WB analysis using 60199-1-Ig (same clone as 60199-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60199-1-Ig (STAT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60199-1-Ig (same clone as 60199-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60199-1-Ig (STAT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60199-1-Ig (same clone as 60199-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60199-1-Ig (STAT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 60199-1-Ig (same clone as 60199-1-PBS)

  IP result of anti-STAT3 (IP:60199-1-Ig, 5ug; Detection:60199-1-Ig 1:2000) with HeLa cells lysate 1400ug. This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:0 (under 10x lens). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:0 (under 40x lens). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:0 (under 10x lens). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:0 (under 40x lens). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60199-1-Ig(STAT3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STAT3 antibody (60199-1-Ig, Clone: 3G2D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60199-1-PBS targets STAT3 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: STAT3 fusion protein Ag0360
• Full Name: signal transducer and activator of transcription 3 (acute-phase response factor)
• Calculated Molecular Weight: 770 aa, 88 kDa
• Observed Molecular Weight: 70-88 kDa
• GenBank Accession Number: BC000627
• Gene Symbol: STAT3
• Gene ID (NCBI): 6774
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a mouse monoclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.