STAT3 Polyclonal antibody

STAT3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 51076-2-AP

Synonyms

STAT 3, HIES, APRF, Acute-phase response factor

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Validation Data Gallery

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  WB analysis using 51076-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51076-2-AP (STAT3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 51076-2-AP

  IP result of anti-STAT3 (IP:51076-2-AP, 4ug; Detection:51076-2-AP 1:500) with HeLa cells lysate 1500ug.

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  IHC staining of human cervical cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51076-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51076-2-AP (STAT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51076-2-AP (STAT3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51076-2-AP (STAT3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 51076-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51076-2-AP (STAT3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cervical cancer tissue, human breast cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IP: See 1 publications below

Product Information

51076-2-AP targets STAT3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: signal transducer and activator of transcription 3 (acute-phase response factor)
• Calculated Molecular Weight: 770 aa, 88 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC000627
• Gene Symbol: STAT3
• Gene ID (NCBI): 6774
• RRID: AB_2198610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.

Publications

Species	Application	Title
mouse	WB	FASEB J; Lmo4-resistin signaling contributes to adipose tissue-liver crosstalk upon weight cycling.; Authors - Yu Sun
mouse	WB	Neurotox Res; Hispidulin Inhibits Neuroinflammation in Lipopolysaccharide-Activated BV2 Microglia and Attenuates the Activation of Akt, NF-κB, and STAT3 Pathway.; Authors - Chung-I Yu
human	WB	Oncol Lett; ST8SIA1 inhibits the proliferation, migration and invasion of bladder cancer cells by blocking the JAK/STAT signaling pathway.; Authors - Shengjin Yu
human	IP	Cell Biol Int; Identification and analysis of glycogen synthase kinase 3 beta1 interactome.; Authors - Gao Xuejuan X
		Mol Med Rep; A small GTPase?like protein fragment of Mycoplasma promotes tumor cell migration and proliferation in?vitro via interaction with Rac1 and Stat3.; Authors - Hu Xiaopeng X
mouse	WB	J Ethnopharmacol; Cyberpharmacology uncover the mechanism of the total Rhizoma Coptidis extracts ameliorate chronic atrophic gastritis; Authors - Zhengcai Ma