STAT1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

STAT1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F7C6

Cat no : 66545-1-PBS

Synonyms

ISGF 3, STAT1, STAT91

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Validation Data Gallery

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  WB analysis using 66545-1-Ig (same clone as 66545-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66545-1-Ig (STAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66545-1-PBS in a different storage buffer formulation.

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  WB analysis using 66545-1-Ig (same clone as 66545-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66545-1-Ig (STAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66545-1-PBS in a different storage buffer formulation.

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  WB analysis using 66545-1-Ig (same clone as 66545-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66545-1-Ig (STAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66545-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66545-1-Ig (same clone as 66545-1-PBS)

  WB result of STAT1 antibody (66545-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66545-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66545-1-Ig (same clone as 66545-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66545-1-Ig (STAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66545-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66545-1-Ig (same clone as 66545-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66545-1-Ig (STAT1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66545-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66545-1-Ig (same clone as 66545-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66545-1-Ig (STAT1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66545-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66545-1-Ig (same clone as 66545-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66545-1-Ig (STAT1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66545-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66545-1-Ig (same clone as 66545-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66545-1-Ig (STAT1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66545-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66545-1-Ig (same clone as 66545-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STAT1 antibody (66545-1-Ig, Clone: 1F7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66545-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66545-1-Ig (same clone as 66545-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed IFN alpha treated HeLa cells using STAT1 antibody (66545-1-Ig, Clone: 1F7C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66545-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66545-1-PBS targets STAT1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: STAT1 fusion protein Ag0199
• Full Name: signal transducer and activator of transcription 1, 91kDa
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC002704
• Gene Symbol: STAT1
• Gene ID (NCBI): 6772
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

What is the molecular weight of STAT1? Are there any isoforms of STAT1? Is STAT1 post-translationally modified?

The molecular weight of STAT1 is 91 kDa for full-length STAT1α and 84 kDa for STAT1β, which is devoid of the C-terminal activation domain (PMID: 1502203). Phosphorylation of STAT1 regulates STAT1 activity. Phosphorylation of Tyr701 by Janus kinases (JAKs) triggers STAT1 homo- and hetero-dimerization with other STAT family members and also causes nuclear translocation, while phosphorylation of Ser727 is needed for full activation (PMID: 10851062). STAT1β lacks Ser727 and has therefore been considered to be inactive or acting in a dominant negative manner to STAT1α, although it has been further shown to be capable of transcription activation in vivo (PMID: 24710278).

 What is the subcellular localization of STAT1?

STAT1 is present in the cytoplasm and in the nucleus. Translocation of STAT1 from the cytoplasm to the nucleus is required for its regulation of gene expression and is dependent on phosphorylation (see above). However, low levels of unphosphorylated STAT1, often referred to as U-STAT1, can also be found in the nucleus, where U-STAT1 regulates the stability of heterochromatin by binding to chromatin in monomeric or dimeric states (PMID: 18840523).

 What is the tissue expression pattern of STAT1?

STAT1 is ubiquitously expressed.