STAM Monoclonal antibody

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  WB analysis using 68628-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68628-1-Ig (STAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of RAW 264.7 using 68628-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68628-1-Ig (STAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

STAM Monoclonal Antibody for WB, ELISA

Cat No. 68628-1-Ig

Clone No.1F5C2

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

DKFZp686J2352, STAM, STAM 1, STAM1

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Tested Applications

• Positive WB detected in: HCT 116 cells, RAW 264.7 cells, HeLa cells, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68628-1-Ig targets STAM in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: STAM fusion protein Ag33192
• Full Name: signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC030586
• Gene Symbol: STAM
• Gene ID (NCBI): 8027
• RRID: AB_3085320
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92783
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAM, also named as STAM1, belongs to the STAM family. It is involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. STAM may also play a role in T-cell development. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs). Together with HRS, STAM forms ESCRT-0. STAM was originally identified as an adaptor protein involved in cytokine signaling.(PMID:20505072)