STAG3 Polyclonal antibody

STAG3 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 23314-1-AP

Synonyms

Cohesin subunit SA 3, SCC3 homolog 3, STAG3, stromal antigen 3, Stromalin 3

---

Validation Data Gallery

• 

  IP experiment of human testis using 23314-1-AP

  IP result of anti-STAG3 (IP:23314-1-AP, 4ug; Detection:23314-1-AP 1:300) with human testis tissue lysate 4000ug.

• 

  IHC staining of human testis using 23314-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23314-1-AP (STAG3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 23314-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23314-1-AP (STAG3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 23314-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23314-1-AP (STAG3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 23314-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23314-1-AP (STAG3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 23314-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using 23314-1-AP (STAG3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 23314-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 23314-1-AP (STAG3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive IP detected in: human testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells, MCF-7 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 23314-1-AP is STAG3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STAG3 fusion protein Ag19818
• Full Name: stromal antigen 3
• Calculated Molecular Weight: 1225 aa, 139 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC146806
• Gene Symbol: STAG3
• Gene ID (NCBI): 10734
• RRID: AB_2879253
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Front Oncol; The Ectopic Expression of Meiosis Regulatory Genes in Cutaneous T-Cell Lymphomas (CTCL).; Authors - Jennifer Gantchev
human	IF	Front Genet; In-Frame Variants in STAG3 Gene Cause Premature Ovarian Insufficiency.; Authors - Wen-Juan Xiao