ST8SIA3 Polyclonal antibody, PBS Only

ST8SIA3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18004-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 18004-1-AP (same clone as 18004-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18004-1-AP (ST8SIA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18004-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 18004-1-AP (same clone as 18004-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18004-1-AP (ST8SIA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18004-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 18004-1-AP (same clone as 18004-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18004-1-AP (ST8SIA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18004-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 18004-1-AP (same clone as 18004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 18004-1-AP (ST8SIA3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18004-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 18004-1-AP (same clone as 18004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 18004-1-AP (ST8SIA3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18004-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 18004-1-AP (same clone as 18004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18004-1-AP (ST8SIA3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18004-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 18004-1-AP (same clone as 18004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18004-1-AP (ST8SIA3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18004-1-PBS in a different storage buffer formulation.

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Product Information

18004-1-PBS targets ST8SIA3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ST8SIA3 fusion protein Ag12497
• Full Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3
• Calculated Molecular Weight: 380 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC074909
• Gene Symbol: ST8SIA3
• Gene ID (NCBI): 51046
• RRID: AB_2239838
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The ST8 α-N-acetyl-neuraminide α-2,8-sialyltransferase (ST8Sia) family catalyzes the synthesis of one or multiple α2,8-linked sialic acid chains according to their acceptor specifificity (PMID:19888308). At least fifive ST8Sia enzymes (ST8SIA1, 2, 3, 4, and 6) have previously been identifified in adult brains (PMID:11530204). The ST8SIA3 is enriched in the striatum. The mouse St8sia3 gene shares 96% amino acid identity with human ST8SIA3, and is mainly expressed in the brain and testis (PMID:31455764).