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ST8SIA3 Polyclonal antibody

ST8SIA3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18004-1-AP

Synonyms

Alpha 2,8 sialyltransferase 8C, Sialyltransferase 8C, Sialytransferase St8Sia III, SIAT8 C, SIAT8C, ST8SIA3, ST8SiaIII

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ST8SIA3 Polyclonal antibody (18004-1-AP)

    WB analysis using 18004-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18004-1-AP (ST8SIA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ST8SIA3 Polyclonal antibody (18004-1-AP)

    WB analysis of mouse liver using 18004-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18004-1-AP (ST8SIA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ST8SIA3 Polyclonal antibody (18004-1-AP)

    WB analysis of mouse liver using 18004-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18004-1-AP (ST8SIA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using ST8SIA3 Polyclonal antibody (18004-1-AP)

    IHC staining of human liver using 18004-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 18004-1-AP (ST8SIA3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ST8SIA3 Polyclonal antibody (18004-1-AP)

    IHC staining of human liver using 18004-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 18004-1-AP (ST8SIA3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using ST8SIA3 Polyclonal antibody (18004-1-AP)

    IHC staining of human skin using 18004-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18004-1-AP (ST8SIA3 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using ST8SIA3 Polyclonal antibody (18004-1-AP)

    IHC staining of human skin using 18004-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18004-1-AP (ST8SIA3 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inA549 cells, mouse liver tissue, Caco-2 cells
Positive IHC detected inhuman liver tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18004-1-AP targets ST8SIA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ST8SIA3 fusion protein Ag12497
Full Name ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3
Calculated Molecular Weight 380 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC074909
Gene Symbol ST8SIA3
Gene ID (NCBI) 51046
RRIDAB_2239838
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ST8 α-N-acetyl-neuraminide α-2,8-sialyltransferase (ST8Sia) family catalyzes the synthesis of one or multiple α2,8-linked sialic acid chains according to their acceptor specifificity (PMID:19888308). At least fifive ST8Sia enzymes (ST8SIA1, 2, 3, 4, and 6) have previously been identifified in adult brains (PMID:11530204). The ST8SIA3 is enriched in the striatum. The mouse St8sia3 gene shares 96% amino acid identity with human ST8SIA3, and is mainly expressed in the brain and testis (PMID:31455764).

Protocols

Product Specific Protocols
WB protocol for ST8SIA3 antibody 18004-1-APDownload protocol
IHC protocol for ST8SIA3 antibody 18004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols