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ST6GAL1 Monoclonal antibody, PBS Only

ST6GAL1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F11G5

Cat no : 68633-1-PBS

Synonyms

CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, CD75, Beta-galactoside alpha-2,6-sialyltransferase 1, B-cell antigen CD75, Alpha 2,6-ST 1

Validation Data Gallery

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  • Western Blot (WB) analysis of TF-1 cells using ST6GAL1 Monoclonal antibody (68633-1-Ig)

    WB analysis of TF-1 using 68633-1-Ig (same clone as 68633-1-PBS)

    TF-1 cells were subjected to SDS PAGE followed by western blot with 68633-1-Ig (ST6GAL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68633-1-PBS in a different storage buffer formulation.

Product Information

68633-1-PBS targets ST6GAL1 in WB, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ST6GAL1 fusion protein Ag6181
Full Name ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 43-45 kDa, 50-70 kDa
GenBank Accession NumberBC040009
Gene Symbol ST6GAL1
Gene ID (NCBI) 6480
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ST6GAL1 (β-galactoside α-2-6 sialyl transferase1; also known as ST6N or CD75) is a sialyltransferase mediating the glycosylation of proteins and lipids to form functionally important glycoproteins and glycolipids in the Golgi compartment. It is principally expressed in liver, placenta and skeletal muscle. ST6GAL1 undergoes proteolytic process to generate soluble form from membrane form. Western blot analysis of human liver using this antibody detects both isoforms between 43-50 kDa. Higher molecular weight of bands around 50-70 kDa can also be observed with unknown reason. (PMID: 15049997)