IL-1RL1/ST2 Polyclonal antibody

IL-1RL1/ST2 Polyclonal Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 30194-1-AP

Synonyms

IL1RL1, IL-33R, IL-1RL1, FIT 1, EC:3.2.2.6

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Validation Data Gallery

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  WB analysis using 30194-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30194-1-AP (IL-1RL1/ST2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  FC experiment of HEK-293 using 30194-1-AP

  1x10^6 HEK-293 cells were surface stained with 0.4 ug IL-1RL1/ST2 Polyclonal antibody (30194-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (blue). Cells were not fixed.

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Tested Applications

• Positive WB detected in: HUVEC cells, human placenta tissue
• Positive FC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30194-1-AP targets IL-1RL1/ST2 in WB, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 1 receptor-like 1
• Calculated Molecular Weight: 63 kDa, 37 kDa, 30 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC030975
• Gene Symbol: IL1RL1
• Gene ID (NCBI): 9173
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST2, also known as IL1RL1, is a member of the IL-1 superfamily and serves as the receptor for IL-33. It plays a major role in immune and inflammatory responses. ST2 is expressed by various types of immune cells including Th2 cells, mast cells, type 2 innate lymphoid cells, eosinophils, basophils, NK cells, and non-immune cells, including cardiomyocytes. Two forms of ST2 exist: a membrane-bound form and a soluble form (sST2). The membrane-bound form activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 acts as a decoy receptor. (PMID: 27055914; 23999434; 28484466)