ST13 Polyclonal antibody

ST13 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26581-1-AP

Synonyms

AAG2, FAM10A1, FAM10A4, HIP, HOP, Hsc70 interacting protein, HSPABP, HSPABP1, P48, PRO0786, Protein FAM10A1, Putative tumor suppressor ST13, SNC6, ST13

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Validation Data Gallery

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  WB analysis of HEK-293 using 26581-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26581-1-AP (ST13 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 26581-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26581-1-AP (ST13 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 26581-1-AP

  IP result of anti-ST13 (IP:26581-1-AP, 4ug; Detection:26581-1-AP 1:2000) with HEK-293 cells lysate 1800ug.

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  IHC staining of human colon cancer using 26581-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26581-1-AP (ST13 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26581-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26581-1-AP (ST13 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse testis tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 26581-1-AP is ST13 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ST13 fusion protein Ag24779
• Full Name: suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein)
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC139724
• Gene Symbol: ST13
• Gene ID (NCBI): 6767
• RRID: AB_2880560
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Sci Rep; Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.; Authors - Katsutaka Mitachi
mouse	WB	Cancer Res; N-myc-Mediated Translation Control Is a Therapeutic Vulnerability in Medulloblastoma; Authors - Duygu Kuzuoglu-Ozturk