SSX4 Polyclonal antibody

SSX4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15876-1-AP

Synonyms

Cancer/testis antigen 5.4, CT5.4, Protein SSX4, SSX4, SSX4A, SSX4B

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Validation Data Gallery

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  WB analysis of MCF-7 using 15876-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15876-1-AP (SSX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 15876-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15876-1-AP (SSX4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 15876-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15876-1-AP (SSX4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 15876-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15876-1-AP (SSX4 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: MCF7 cells, A549 cells, K-562 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15876-1-AP targets SSX4 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SSX4 fusion protein Ag8637
• Full Name: synovial sarcoma, X breakpoint 4
• Calculated Molecular Weight: 188 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC005325
• Gene Symbol: SSX4
• Gene ID (NCBI): 6759
• RRID: AB_2035018
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.