SSTR1 Polyclonal antibody

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  WB analysis of L02 using 20587-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20587-1-AP (SSTR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 20587-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20587-1-AP (SSTR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 20587-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20587-1-AP (SSTR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20587-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20587-1-AP (SSTR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 20587-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20587-1-AP (SSTR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  FC experiment of SH-SY5Y using 20587-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug SSTR1 Polyclonal antibody (20587-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

SSTR1 Polyclonal Antibody for WB, ELISA, FC (Intra)

Cat No. 20587-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

SS1 R, SS 1 R, SRIF-2, SRIF 2, Somatostatin receptor type 1

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Tested Applications

• Positive WB detected in: L02 cells, BxPC-3 cells, mouse brain tissue, mouse small intestine tissue, SH-SY5Y cells
• Positive FC (Intra) detected in: SH-SY5Y cells
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

20587-1-AP targets SSTR1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SSTR1 fusion protein Ag14287
• Full Name: somatostatin receptor 1
• Calculated Molecular Weight: 391 aa, 43 kDa
• Observed Molecular Weight: 53 kDa, 63 kDa
• GenBank Accession Number: BC035618
• Gene Symbol: SSTR1
• Gene ID (NCBI): 6751
• RRID: AB_10755146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P30872
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); The Primary Cilia are Associated with the Axon Initial Segment in Neurons; Authors - Han Wang ; KD Validated