SSRP1 Monoclonal antibody

• Featured Product
• KD/KO Validated

SSRP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3D3H4

Cat no : 67313-1-Ig

Synonyms

SSRP1, Structure-specific recognition protein 1, T160, 3D3H4, Chromatin-specific transcription elongation factor 80 kDa subunit, Facilitates chromatin transcription complex 80 kDa subunit, Facilitates chromatin transcription complex subunit SSRP1, FACT, FACT 80 kDa subunit, FACT80, FACTp80, hSSRP1, Recombination signal sequence recognition protein 1

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Validation Data Gallery

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  WB analysis using 67313-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67313-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 67313-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 67313-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 67313-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67313-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67313-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 67313-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67313-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67313-1-Ig (SSRP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 67313-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SSRP1 antibody (67313-1-Ig, Clone: 3D3H4 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, 4T1 cells, NCCIT cells, PC-12 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, LNCaP cells, NIH/3T3 cells
• Positive IHC detected in: human ovary tumor tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

67313-1-Ig targets SSRP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SSRP1 fusion protein Ag8318
• Full Name: structure specific recognition protein 1
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC005116
• Gene Symbol: SSRP1
• Gene ID (NCBI): 6749
• RRID: AB_2882573
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Biomed Pharmacother; CBL0137 activates ROS/BAX signaling to promote caspase-3/GSDME-dependent pyroptosis in ovarian cancer cells; Authors - Chang Yang ; KD Validated