TRAPA/SSR1 Polyclonal antibody

TRAPA/SSR1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 10583-1-AP

Synonyms

SSR1, TRAP alpha, TRAP a, Translocon-associated protein subunit alpha, SSR-alpha

---

Validation Data Gallery

• 

  WB analysis using 10583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 10583-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10583-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 10583-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10583-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 10583-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 10583-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 10583-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TRAPA/SSR1 antibody (10583-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Tested Applications

• Positive WB detected in: COS-7 cells, HepG2 cells, human liver tissue, mouse liver tissue, L02 cells
• Positive IHC detected in: human ovary tumor tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

10583-1-AP targets TRAPA/SSR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples.

• Tested Reactivity: human, mouse, monkey
• Cited Reactivity: human, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAPA/SSR1 fusion protein Ag0878
• Full Name: signal sequence receptor, alpha
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC007710
• Gene Symbol: TRAPA
• Gene ID (NCBI): 6745
• RRID: AB_2195616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).

Publications

Species	Application	Title
human	IF	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
human	WB	Sci Adv; The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.; Authors - Chatchai Phoomak
human	IF	Biosens Bioelectron; Live imaging of RNA and RNA splicing in mammalian cells via the dcas13a-SunTag-BiFC system.; Authors - Mao Chen
human	WB	FASEB J; GPR37 is processed in the N-terminal ectodomain by ADAM10 and furin.; Authors - S Orvokki Mattila
rat	WB,IF	Exp Cell Res; Microdomains of endoplasmic reticulum within the sarcoplasmic reticulum of skeletal myofibers.; Authors - Kaakinen Mika M

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Chatchai (Verified Customer) (09-26-2019)

This anti-SSR1 is very good, presents the specific band at the right size of the protein.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:500 for WB
• Cell Tissue Type: A549, HEK-293