SSH3 Polyclonal antibody

SSH3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18324-1-AP

Synonyms

hSSH 3L, SSH 3, SSH 3L, SSH like protein 3, SSH3, SSH3L

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Validation Data Gallery

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  WB analysis using 18324-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18324-1-AP (SSH3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18324-1-AP

  IP result of anti-SSH3 (IP:18324-1-AP, 4ug; Detection:18324-1-AP 1:1000) with HeLa cells lysate 2320 ug.

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  IHC staining of human colon cancer using 18324-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18324-1-AP (SSH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 18324-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18324-1-AP (SSH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18324-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18324-1-AP (SSH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 18324-1-AP

  Immunofluorescent analysis of A431 cells using 18324-1-AP (SSH3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HT-29 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

18324-1-AP targets SSH3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SSH3 fusion protein Ag13174
• Full Name: slingshot homolog 3 (Drosophila)
• Calculated Molecular Weight: 659 aa, 73 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: BC004210
• Gene Symbol: SSH3
• Gene ID (NCBI): 54961
• RRID: AB_2195490
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC,CoIP	Am J Cancer Res; SSH3 facilitates colorectal cancer cell invasion and metastasis by affecting signaling cascades involving LIMK1/Rac1.; Authors - Yu-Han Hu