SSH2 Polyclonal antibody

SSH2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20674-1-AP

Synonyms

hSSH 2L, KIAA1725, SSH 2, SSH 2L, SSH like protein 2, SSH2, SSH2L

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Validation Data Gallery

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  WB analysis using 20674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20674-1-AP (SSH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 20674-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20674-1-AP (SSH2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 20674-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20674-1-AP (SSH2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, MG-63 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

20674-1-AP targets SSH2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: slingshot homolog 2 (Drosophila)
• Calculated Molecular Weight: 158 kDa
• Observed Molecular Weight: 45-51 kDa
• GenBank Accession Number: NM_033389
• Gene Symbol: SSH2
• Gene ID (NCBI): 85464
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Adh Migr; The modulation of actin dynamics via atypical protein kinase-C activated Cofilin regulates metastasis of colorectal cancer cells.; Authors - S M Anisul Islam