SSBP1 Recombinant antibody

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  WB analysis using 84939-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84939-3-RR (SSBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84939-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84939-3-RR against Human SSBP1 were performed. The affinity constant is 0.144 nM.

SSBP1 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 84939-3-RR

Clone No.242519C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

242519C5, Mt SSB, MtSSB, Mt-SSB, PWP1 interacting protein 17

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Tested Applications

• Positive WB detected in: A375 cells, A549 cells, Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84939-3-RR targets SSBP1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SSBP1 fusion protein Ag2860
• Full Name: single-stranded DNA binding protein 1
• Calculated Molecular Weight: 148 aa, 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC000895
• Gene Symbol: SSBP1
• Gene ID (NCBI): 6742
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q04837
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SSBP1 is a housekeeping gene involved in mitochondrial biogenesis. it binds preferentially and cooperatively to ss-DNA in the maintainance of genome stability. SSBP1 acted as a homotetramer to stabilize the displaced single strand of the normal and expanded displacement loop (D loop) during mtDNA replication, thus preventing formation of secondary single-stranded DNA structures, which could stop the gamma-DNA polymerase.