SSB Polyclonal antibody

SSB Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11720-1-AP

Synonyms

SS B, Lupus La protein, LARP3, La ribonucleoprotein, La autoantigen

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Validation Data Gallery

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  WB analysis of Jurkat using 11720-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11720-1-AP (SSB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11720-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11720-1-AP (SSB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11720-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11720-1-AP (SSB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 11720-1-AP

  IP result of anti-SSB (IP:11720-1-AP, 4ug; Detection:11720-1-AP 1:300) with Jurkat cells lysate 3600ug.

• 

  IF Staining of HeLa using 11720-1-AP

  Immunofluorescent analysis of HeLa cells using 11720-1-AP (SSB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HEK-293 cells
• Positive IP detected in: Jurkat cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

11720-1-AP targets SSB in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SSB fusion protein Ag2294
• Full Name: Sjogren syndrome antigen B (autoantigen La)
• Calculated Molecular Weight: 408 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC001289
• Gene Symbol: SSB
• Gene ID (NCBI): 6741
• RRID: AB_2877790
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	EMBO Rep; Human METTL16 is a N6-methyladenosine (m6A) methyltransferase that targets pre-mRNAs and various non-coding RNAs.; Authors - Ahmed S Warda
human	WB	Oxid Med Cell Longev; The Formation of Melanocyte Apoptotic Bodies in Vitiligo and the Relocation of Vitiligo Autoantigens under Oxidative Stress.; Authors - Jun Tian
human	WB	J Virol; Inhibition of Hepatitis B Virus Gene Expression and Replication by Hepatocyte Nuclear Factor 6.; Authors - Ruidong Hao