SRY Polyclonal antibody

SRY Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17930-1-AP

Synonyms

sex determining region Y, SRY, TDF, TDY, Testis determining factor

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Validation Data Gallery

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  WB analysis of human testis using 17930-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17930-1-AP (SRY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17930-1-AP (SRY antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17930-1-AP (SRY antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17930-1-AP (SRY antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17930-1-AP (SRY antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17930-1-AP (SRY antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17930-1-AP (SRY antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse testis using 17930-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17930-1-AP (SRY antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human testis tissue
• Positive IHC detected in: mouse testis tissue, human testis tissue, human spleen tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17930-1-AP targets SRY in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRY fusion protein Ag12360
• Full Name: sex determining region Y
• Calculated Molecular Weight: 204 aa, 24 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC074924
• Gene Symbol: SRY
• Gene ID (NCBI): 6736
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRY (sex-determining region Y protein) is a tran-scriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.