SRX1 Polyclonal antibody

SRX1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29849-1-AP

Synonyms

SRXN1, Sulfiredoxin-1, Sulfiredoxin 1, SRX, EC:1.8.98.2

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Validation Data Gallery

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  WB analysis using 29849-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29849-1-AP (SRX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 29849-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29849-1-AP (SRX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, PC-12 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29849-1-AP targets SRX1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRX1 fusion protein Ag31537
• Full Name: sulfiredoxin 1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 14KD
• Observed Molecular Weight: 13-16 kDa
• GenBank Accession Number: NM_080725
• Gene Symbol: SRXN1
• Gene ID (NCBI): 140809
• RRID: AB_2918350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.