SRRT Polyclonal antibody

• Featured Product
• KD/KO Validated

SRRT Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20353-1-AP

Synonyms

serrate, ASR2, Arsenite-resistance protein 2, Arsenite resistance protein 2, ARS2

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Validation Data Gallery

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  WB analysis using 20353-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20353-1-AP (SRRT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20353-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20353-1-AP (SRRT antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 20353-1-AP

  WB result of SRRT antibody (20353-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRRT transfected NIH/3T3 cells.

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  IP experiment of Jurkat using 20353-1-AP

  IP result of anti-SRRT (IP:20353-1-AP, 4ug; Detection:20353-1-AP 1:30000) with Jurkat cells lysate 1440 ug.

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  IF Staining of MCF-7 using 20353-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SRRT antibody (20353-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, NIH/3T3 cells, HepG2 cells, K-562 cells
• Positive IP detected in: Jurkat cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20353-1-AP targets SRRT in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRRT fusion protein Ag14196
• Full Name: serrate RNA effector molecule homolog (Arabidopsis)
• Calculated Molecular Weight: 876 aa, 101 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC000082
• Gene Symbol: SRRT
• Gene ID (NCBI): 51593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRRT (Serrate RNA effector molecule homolog) is also named as ARS2 (Arsenite-resistance protein 2). ARS2/SRRT is an essential eukaryotic protein that has emerged as a critical factor in the sorting of functional from non-functional RNA polymerase II (Pol II) transcripts (PMID: 34060620). SRRT protein is a key component of the nuclear cap-binding complex (CBC), which allows it to modulate the expression of various miRNAs. The SRRT protein is widely expressed throughout the eukaryotes, but not in budding yeast. SRRT has been shown to play a vital role in stem-cell renewal, proliferation, hematopoiesis, and RNA-mediated gene silencing and regulation of miRNA biogenesis (PMID: 37345203). SRRT is involved in cellular proliferation and colony formation (tumorigenicity). Similarly, high SRRT expression has been found in cholangiocarcinoma. ARS2 showed ubiquitous expression, with the highest levels in mouse testes, heart, and liver and human heart and skeletal muscle (PMID: 18086880).