SRPK2 Recombinant antibody, PBS Only

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  WB analysis using 85613-5-RR (same clone as 85613-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85613-5-RR (SRPK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85613-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85613-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85613-5-RR against Human SRPK2 were performed. The affinity constant is 4.16 nM.

SRPK2 Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 85613-5-PBS

Clone No.242935B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

EC:2.7.11.1, Serine/arginine-rich protein-specific kinase 2, SFRS protein kinase 2, SFRSK2, SR protein specific kinase 2

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Product Information

85613-5-PBS targets SRPK2 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SRPK2 fusion protein Ag17885
• Full Name: SFRS protein kinase 2
• Calculated Molecular Weight: 688 aa, 78 kDa
• Observed Molecular Weight: 105-110 kDa
• GenBank Accession Number: BC035214
• Gene Symbol: SRPK2
• Gene ID (NCBI): 6733
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78362
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SRPK2, also named as serine/arginine protein kinase 2 and SFRSK2, belongs to SR protein-specific kinase family which phosphorylate the serine/arginine domain containing proteins in the nuclear speckles to mediate pre-mRNA splicing (PMID: 22854045). In addition to its major role as kinase for the phosphorylation of SR proteins, SRPK2 is involved in cell cycle regulation and cell apoptosis (PMID: 19592491). SRPK2 promotes the proliferation and migration of the colon cancer cells. It has been reported to be regulated in several cancer types (PMID: 27041240). It has 2 isoforms with the calculated molecular mass of 77-79 kDa, and the apparent molecular mass of 105-115 kDa due to posttranslational modification (PMID: 12134018, 16122776).