SRPK1 Polyclonal antibody

SRPK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14073-1-AP

Synonyms

SR-protein-specific kinase 1, SR protein specific kinase 1, SFRSK1, SFRS protein kinase 1, Serine/arginine-rich protein-specific kinase 1

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Validation Data Gallery

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  WB analysis using 14073-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14073-1-AP (SRPK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14073-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14073-1-AP (SRPK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14073-1-AP

  IP result of anti-SRPK1 (IP:14073-1-AP, 4ug; Detection:14073-1-AP 1:500) with mouse testis tissue lysate 2040 ug.

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  IHC staining of human breast cancer using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 14073-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14073-1-AP (SRPK1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 14073-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 14073-1-AP (SRPK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, L02 cells, mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human breast cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

14073-1-AP targets SRPK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRPK1 fusion protein Ag5149
• Full Name: SFRS protein kinase 1
• Calculated Molecular Weight: 92.4 kDa
• Observed Molecular Weight: 72-95 kDa
• GenBank Accession Number: BC038292
• Gene Symbol: SRPK1
• Gene ID (NCBI): 6732
• RRID: AB_2194745
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRPK1 (serine-arginine protein kinase 1) is a protein kinase that specifically phosphorylates proteins containing serine-arginine-rich domains. Its substrates include a family of SR proteins that are key regulators of mRNA AS (alternative splicing). SRPK1 may be localized both in the cytoplasm and nucleus and translocates between these compartments under various conditions (e.g. cell cycle and stress). It is able to phosphorylate SR proteins both in cytoplasm and nucleus (the significance of which remains incompletely understood) and thus regulate the activity of these proteins by affecting their ability to bind other proteins and/or RNA as well as their intracellular localization.(PMID:22817743). This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
	WB,CoIP	Cell Metab; DRAK2 aggravates nonalcoholic fatty liver disease progression through SRSF6-associated RNA alternative splicing.; Authors - Yufeng Li
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	J Cancer; Clinical and functional significance of CHK1-S, an alternatively spliced isoform of the CHK1 gene, in hepatocellular carcinoma.; Authors - Guanghui Hu
mouse	IF	BMC Cancer; MiR-659-3p inhibits osteosarcoma progression and metastasis by inhibiting cell proliferation and invasion via targeting SRPK1; Authors - Yubao Gong
human	WB	Nat Commun; LncRNA-LncDACH1 mediated phenotypic switching of smooth muscle cells during neointimal hyperplasia in male arteriovenous fistulas; Authors - Zhaozheng Li ; KD Validated
human	WB	Mol Oncol; CDK12-inactivation-induced MYC signaling causes dependency on the splicing kinase SRPK1; Authors - Jing Liang