SRP54 Recombinant antibody

SRP54 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241125G1

Cat no : 84014-6-RR

Synonyms

Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 241125G1

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Validation Data Gallery

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  WB analysis using 84014-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84014-6-RR (SRP54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84014-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84014-6-RR (SRP54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 84014-6-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84014-6-RR (SRP54 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84014-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84014-6-RR against Human SRP54 were performed. The affinity constant is 4.24 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, RAW 264.7 cells, HEK-293 cells, MCF-7 cells, Jurkat cells, NIH/3T3 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84014-6-RR is SRP54 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SRP54 fusion protein Ag2327
• Full Name: signal recognition particle 54kDa
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC003389
• Gene Symbol: SRP54
• Gene ID (NCBI): 6729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).