SRP54 Recombinant antibody, PBS Only (Detector)

SRP54 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241125D6

Cat no : 84014-5-PBS

Synonyms

Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 241125D6

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Validation Data Gallery

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  WB analysis using 84014-5-RR (same clone as 84014-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84014-5-RR (SRP54 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 84014-5-RR (same clone as 84014-5-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84014-5-RR (SRP54 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00955-4

  Sandwich ELISA standard curve of MP00955-4, Human SRP54 Recombinant Matched Antibody Pair - PBS only. 84014-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2327. 84014-5-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP00955-4

  The mean SRP54 concentration was determined to be 124.39 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load.

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  IF Staining of HeLa using 84014-5-RR (same clone as 84014-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SRP54 antibody (84014-5-RR, Clone: 241125D6 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84014-5-RR (same clone as 84014-5-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Srp54 Recombinant Antibody (84014-5-RR, Clone:241125D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84014-5-RR (same clone as 84014-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Srp54 Recombinant Antibody (84014-5-RR, Clone:241125D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84014-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84014-5-RR against Human SRP54 were performed. The affinity constant is 0.287 nM.

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Product Information

84014-5-PBS targets SRP54 as part of a matched antibody pair:

MP00955-4: 84014-2-PBS capture and 84014-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SRP54 fusion protein Ag2327
• Full Name: signal recognition particle 54kDa
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC003389
• Gene Symbol: SRP54
• Gene ID (NCBI): 6729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).