SRGAP3 Polyclonal antibody

SRGAP3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 29624-1-AP

Synonyms

SRGAP 3, SLIT-ROBO Rho GTPase-activating protein 3, SLIT ROBO Rho GTPase activating protein 3, Rho GTPase-activating protein 14, Mental disorder-associated GAP

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Validation Data Gallery

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  WB analysis using 29624-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29624-1-AP (SRGAP3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 29624-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29624-1-AP (SRGAP3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 29624-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SRGAP3 antibody (29624-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29624-1-AP targets SRGAP3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRGAP3 fusion protein Ag31196
• Full Name: SLIT-ROBO Rho GTPase activating protein 3
• Calculated Molecular Weight: 124 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: NM_014850
• Gene Symbol: SRGAP3
• Gene ID (NCBI): 9901
• RRID: AB_2918334
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRGAP3 (SLIT-ROBO Rho GTPase Activating Protein 3), also named MEGAP and WRP, is a member of the Slit-Robo sub-family of Rho GTPase-activating proteins (Rho GAPs), controls actin and microtubule dynamics through negative regulation of Rac(PMID: 23108406). Diseases associated with SRGAP3 include Pilomyxoid Astrocytoma and Avoidant Personality Disorder. SRGAP3 is highly expressed in fetal and adult brain, including the cortex and the hippocampus.