SRGAP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SRGAP2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA, IF

Conjugate

Unconjugated

Publications

4

Cat no : 22519-1-AP

Synonyms

SRGAP2A, SLIT-ROBO Rho GTPase-activating protein 2, Rho GTPase-activating protein 34, Formin-binding protein 2, FNBP2

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Validation Data Gallery

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  WB analysis of mouse kidney using 22519-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22519-1-AP (SRGAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22519-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22519-1-AP (SRGAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 22519-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22519-1-AP (SRGAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 22519-1-AP

  IP result of anti-SRGAP2 (IP:22519-1-AP, 4ug; Detection:22519-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

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  IHC staining of human kidney using 22519-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22519-1-AP (SRGAP2 Antibody) at dilution of 1:50.

• 

  IHC staining of mouse brain using 22519-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22519-1-AP (SRGAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: mouse brain tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

22519-1-AP targets SRGAP2 in WB, IHC, IP, ELISA, IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRGAP2 fusion protein Ag18278
• Full Name: SLIT-ROBO Rho GTPase activating protein 2
• Calculated Molecular Weight: 1071 aa, 121 kDa
• Observed Molecular Weight: 121 kDa
• GenBank Accession Number: BC132874
• Gene Symbol: SRGAP2
• Gene ID (NCBI): 23380
• RRID: AB_2879114
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLIT-ROBO Rho GTPase-activating protein 2 (SRGAP2) is also named as ARHGAP34, FNBP2, KIAA0456 and SRGAP2A. SRGAP2 plays important roles in axon guidance, neuronal migration, synapse formation, and nerve regeneration. SRGAP2 protein was first expressed in the retina of normal mice at the embryonic stage and was mainly located in the mature retinal ganglion cell layer and the inner nuclear layer (PMID: 37056152). SRGAP2 plays a key role in neuronal morphogenesis and migration mainly during development of the cerebral cortex (PMID: 20810653, PMID: 27373832, PMID: 28333212). SRGAP2 are critical for neuronal migration through inactivation of Rho GTPases Cdc42, Rac1, and RhoA (PMID: 20810653). SRGAP2A promotes synaptic maturation, and ultimately the synaptic accumulation of AMPA and GABAA receptors, by interacting with key components of both excitatory and inhibitory postsynaptic scaffolds, Homer and Gephyrin (PMID: 27373832).

Publications

Species	Application	Title
human	WB,IHC,IF	Theranostics; Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer; Authors - Chen Li ; KD Validated
human	WB	Nat Commun; Haploinsufficiency in the ANKS1B gene encoding AIDA-1 leads to a neurodevelopmental syndrome.; Authors - Abigail U Carbonell
mouse	WB	Cell Rep; Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeleton; Authors - Doris Wennagel
mouse	WB,IHC	Biomed Pharmacother; CDDO-Me ameliorates podocyte injury through anti-oxidative stress and regulation of actin cytoskeleton in adriamycin nephropathy; Authors - Cheng Xu