|Positive WB detected in||HUVEC cells, HeLa cells, HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Sample-dependent, check data in validation data gallery|
28212-1-AP targets SREBF2 in WB, IHC, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse, hamster|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SREBF2 fusion protein Ag28205|
|Full Name||sterol regulatory element binding transcription factor 2|
|Calculated molecular weight||124 kDa|
|Observed molecular weight||73-75 kDa, 120-130 kDa|
|GenBank accession number||BC056158|
|Gene ID (NCBI)||6721|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis. SREBF2 expression is dynamically regulated in response to nutritional and hormonal cues. SREBF2 exists two isoform with molecular weight 124 kDa and 73 kDa.
Lipophagy-mediated cholesterol synthesis inhibition is required for the survival of hepatocellular carcinoma under glutamine deprivation
CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.
Front Cell Dev Biol
Brahma Related Gene 1 (Brg1) Regulates Cellular Cholesterol Synthesis by Acting as a Co-factor for SREBP2.
Diabetes Metab J
Long Non-Coding RNA TUG1 Attenuates Insulin Resistance in Mice with Gestational Diabetes Mellitus via Regulation of the MicroRNA-328-3p/SREBP-2/ERK Axis
Cancer Commun (Lond)
PTPRO represses colorectal cancer tumorigenesis and progression by reprogramming fatty acid metabolism.
Front Cell Dev Biol
The Chromatin Remodeling Protein BRG1 Regulates SREBP Maturation by Activating SCAP Transcription in Hepatocytes.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (04-17-2023)
OK for WB, a specific but weak band was seen around 150 kd, but not 130 kd, which is shown on its booklet. The 130 kd band is a strong nonspecific band.