CoraLite®594-conjugated SREBF1 Monoclonal antibody

SREBF1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B6G5

Cat no : CL594-66875

Synonyms

bHLHd1, SREBF1, SREBP 1, SREBP 1c, SREBP1

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66875

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 SREBF1 antibody (CL594-66875, Clone: 1B6G5 ) at dilution of 1:100. Green: stain with CoraLite®488-Phalloidin (CatNo. PF00001).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66875 targets SREBF1 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SREBF1 fusion protein Ag5484
• Full Name: sterol regulatory element binding transcription factor 1
• Calculated Molecular Weight: 1177 aa, 125 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: BC063281
• Gene Symbol: SREBF1
• Gene ID (NCBI): 6720
• RRID: AB_2920050
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267]. This antibody can recognize the 125kd precursor form and the 68kd mature form of human SREBF1.