SRA1 Polyclonal antibody

SRA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 24655-1-AP

Synonyms

Steroid receptor RNA activator protein, Steroid receptor RNA activator 1, SRAP, PP7684

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Validation Data Gallery

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  WB analysis of A2780 using 24655-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 24655-1-AP (SRA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24655-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24655-1-AP (SRA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 24655-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 24655-1-AP (SRA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 24655-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24655-1-AP (SRA1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 24655-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24655-1-AP (SRA1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse testis using 24655-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24655-1-AP (SRA1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24655-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24655-1-AP (SRA1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 24655-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SRA1 antibody (24655-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  FC experiment of MCF-7 using 24655-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug SRA1 Polyclonal antibody (24655-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A2780 cells, HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 1 publications below

Product Information

24655-1-AP targets SRA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SRA1 fusion protein Ag20082
• Full Name: steroid receptor RNA activator 1
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 30 kDa, 33 kDa
• GenBank Accession Number: BC067895
• Gene Symbol: SRA1
• Gene ID (NCBI): 10011
• RRID: AB_2879657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Steroid receptor RNA activator 1 (SRA1), also known as seroid receptor RNA activator protein (SRAP) or SRA, is involved in modulating the activity of multiple transcription factors including the estrogen receptor (ER) (PMID: 20398657). It may play a role in tumorigenesis (PMID: 16152589). The SRA1 gene encodes both SRA1 protein and a non-coding functional RNA that functions as part of a ribonucleoprotein complex activating steroid receptor induced transcription. This polyclonal antibody recognizes endogenous SRA1 isoforms which migrate as a doublet on SDS-PAGE gels (PMID: 12565891; 23907597).

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; Up-regulation of thioredoxin system by puerarin inhibits lipid uptake in macrophages; Authors - Wenchao Li,
human,mouse	WB	Phytomedicine; Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux; Authors - Jianfei Mu
human, mouse	WB	Arterioscler Thromb Vasc Biol; RP5-833A20.1/miR-382-5p/NFIA-Dependent Signal Transduction Pathway Contributes to the Regulation of Cholesterol Homeostasis and Inflammatory Reaction.; Authors - Yan-Wei Hu
human	WB	J Lipid Res; A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent Signal Transduction Pathway Is Essential for the Regulation of Cholesterol Homeostasis and Inflammatory Reactions.; Authors - Yan-Wei Hu
human	WB	J Lipid Res; VNN1 Promotes Atherosclerosis Progression in apoE Deficiency Mice Fed a High Fat High Cholesterol Diet.; Authors - Yan-Wei Hu
human	WB	PLoS One; Dihydrocapsaicin Attenuates Plaque Formation through a PPARγ/LXRα Pathway in apoE(-/-) Mice Fed a High-Fat/High-Cholesterol Diet.; Authors - Hu Yan-Wei YW