P62/SQSTM1 Polyclonal antibody

• Featured Product
• KD/KO Validated

P62/SQSTM1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 55274-1-AP

Synonyms

P62, P62/SQSTM1, sequestosome 1, SQSTM1, Ubiquitin binding protein p62

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Validation Data Gallery

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  WB analysis using 55274-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55274-1-AP (P62/SQSTM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 55274-1-AP

  WB result of P62/SQSTM1 antibody (55274-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62/SQSTM1 transfected HEK-293 cells.

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  WB analysis of MCF-7 using 55274-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55274-1-AP (SQSTM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of U2OS using 55274-1-AP

  U2OS lysates prepared and IP of SQSTM1 performed using 1.0 μg of 55274-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human breast cancer using 55274-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55274-1-AP (SQSTM1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 55274-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55274-1-AP (SQSTM1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 55274-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55274-1-AP (P62/SQSTM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 55274-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55274-1-AP (P62/SQSTM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 55274-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55274-1-AP (P62/SQSTM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 55274-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55274-1-AP (P62/SQSTM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 55274-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55274-1-AP (SQSTM1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 55274-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55274-1-AP (SQSTM1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 55274-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 55274-1-AP (P62/SQSTM1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 55274-1-AP

  U2OS WT cells (yellow outline) and SQSTM1 KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 55274-1-AP at 1:300. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, HepG2 cells
• Positive IP detected in: U2OS cells
• Positive IHC detected in: human breast cancer tissue, mouse brain tissue, mouse kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 22 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

55274-1-AP targets P62/SQSTM1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: sequestosome 1
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_003900
• Gene Symbol: P62/SQSTM1
• Gene ID (NCBI): 8878
• RRID: AB_11182278
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SQSTM1, also named as ORCA, OSIL, p60, EBIAP, A170, PDB3, ZIP3, p62 and p62B, is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. It may play a role in titin/TTN downstream signaling in muscle cells. SQSTM1 may regulate signaling cascades through ubiquitination. It is an adapter that mediates the interaction between TRAF6 and CYLD. SQSTM1 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels (PMID: 10356400). This antibody is specific to SQSTM1.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Autophagy-mediated apoptosis eliminates aneuploid cells in a mouse model of chromosome mosaicism.; Authors - Shruti Singla
human	WB	Cell Death Differ; CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation.; Authors - Yina Liao
human	WB	Autophagy; An integrative multi-omics approach uncovers the regulatory role of CDK7 and CDK4 in autophagy activation induced by silica nanoparticles.; Authors - Chen Ruan
mouse	WB,IF	Front Pharmacol; Morroniside Inhibits H2O2-Induced Podocyte Apoptosis by Down-Regulating NOX4 Expression Controlled by Autophagy In Vitro.; Authors - Xue Gao
human,mouse	WB	Front Pharmacol; Resolvin D1 attenuates CCl4 Induced Liver Fibrosis by Inhibiting Autophagy-Mediated HSC activation via AKT/mTOR Pathway; Authors - Jiahuan Li
mouse	WB	Nutrients; Punicalagin Prevents Inflammation in LPS-Induced RAW264.7 Macrophages by Inhibiting FoxO3a/Autophagy Signaling Pathway.; Authors - Yuan Cao