SQLE Monoclonal antibody, PBS Only

SQLE Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C9A2

Cat no : 67206-1-PBS

Synonyms

ERG1, FLJ30795, SE, SQLE, squalene epoxidase, Squalene monooxygenase

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Validation Data Gallery

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  WB analysis of A549 using 67206-1-Ig (same clone as 67206-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67206-1-Ig (same clone as 67206-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67206-1-Ig (same clone as 67206-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67206-1-Ig (same clone as 67206-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 67206-1-Ig (same clone as 67206-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 67206-1-Ig (same clone as 67206-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SQLE antibody (67206-1-Ig, Clone: 1C9A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

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Product Information

67206-1-PBS targets SQLE in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Rat, pig samples.

• Tested Reactivity: Human, Rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SQLE fusion protein Ag3266
• Full Name: squalene epoxidase
• Calculated Molecular Weight: 574 aa, 64 kDa
• Observed Molecular Weight: 50-64 kDa
• GenBank Accession Number: BC017033
• Gene Symbol: SQLE
• Gene ID (NCBI): 6713
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation. SQLE is subject to feedback regulation via cholesterol-induced degradation, which depends on its lipid-sensing N terminal regulatory domain. Truncation of SQLE occurs during its endoplasmic reticulum-associated degradation and requires the proteasome, which partially degrades the SQLE N-terminus and eliminates cholesterol-sensing elements within this region. The MW of SQLE is about 50-64 kDa. (PMID:21356516, PMID: 28972164)