SPZ1 Polyclonal antibody

SPZ1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18931-1-AP

Synonyms

NYD TSP1, spermatogenic leucine zipper 1, SPZ1, Testis specific protein 1, TSP1

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Validation Data Gallery

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  WB analysis of PC-3 using 18931-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18931-1-AP (SPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 18931-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 18931-1-AP (SPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 18931-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 18931-1-AP (SPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 18931-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of PC-3 using 18931-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 18931-1-AP (SPZ1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells, DU 145 cells, human testis tissue
• Positive IHC detected in: human testis tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18931-1-AP targets SPZ1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPZ1 fusion protein Ag6995
• Full Name: spermatogenic leucine zipper 1
• Calculated Molecular Weight: 430 aa, 49 kDa
• Observed Molecular Weight: 49-55 kDa
• GenBank Accession Number: BC033798
• Gene Symbol: SPZ1
• Gene ID (NCBI): 84654
• RRID: AB_2195884
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.