SPZ1 Polyclonal antibody

SPZ1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18931-1-AP

Synonyms

NYD TSP1, spermatogenic leucine zipper 1, SPZ1, Testis specific protein 1, TSP1



Tested Applications

Positive WB detected inPC-3 cells, DU 145 cells, human testis tissue
Positive IHC detected inhuman testis tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18931-1-AP targets SPZ1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPZ1 fusion protein Ag6995
Full Name spermatogenic leucine zipper 1
Calculated Molecular Weight 430 aa, 49 kDa
Observed Molecular Weight 49-55 kDa
GenBank Accession NumberBC033798
Gene Symbol SPZ1
Gene ID (NCBI) 84654
RRIDAB_2195884
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SPZ1 antibody 18931-1-APDownload protocol
IHC protocol for SPZ1 antibody 18931-1-APDownload protocol
IF protocol for SPZ1 antibody 18931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols