SPTLC3 Polyclonal antibody

SPTLC3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16600-1-AP

Synonyms

Long chain base biosynthesis protein 3, Long chain base biosynthesis protein 2b, LCB2b, LCB 3, EC:2.3.1.50

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Validation Data Gallery

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  WB analysis using 16600-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16600-1-AP (SPTLC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 16600-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16600-1-AP (SPTLC3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16600-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SPTLC3 antibody (16600-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16600-1-AP targets SPTLC3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPTLC3 fusion protein Ag9760
• Full Name: serine palmitoyltransferase, long chain base subunit 3
• Calculated Molecular Weight: 175 aa, 20 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC020656
• Gene Symbol: SPTLC3
• Gene ID (NCBI): 55304
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serine-palmitoyltransferase (SPT) is a pyridoxal 5‐phosphate (PLP)-dependent α‐oxoaminotransferase that consists of three core subunits-SPTLC1, SPTLC2, and SPTLC3-that share a mutual homology. The amino acid sequences between SPTLC1 and SPTLC2 (or SPTLC3) show a mutual similarity of about 20%, whereas the sequences for SPTLC2 and SPTLC3 show a significantly higher identity of 68% (with 84% similarity) on the amino acid level. SPTLC3 is not or only little expressed in tissues that are functionally connected to erythropoiesis or immune response like peripheral blood cells, bone marrow, and spleen. Instead, the lack of SPTLC3 expression seems to be compensated by increased expression of SPTLC2 in these tissues. (PMID: 17331073, PMID: 17023427)

Publications

Species	Application	Title
human	WB	Am J Cancer Res; TRIM8 inhibits breast cancer proliferation by regulating estrogen signaling.; Authors - Zelin Tian