SPTLC1 Monoclonal antibody, PBS Only (Detector)

• Featured Product
• KD/KO Validated

SPTLC1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D11D7

Cat no : 66899-1-PBS

Synonyms

Serine-palmitoyl-CoA transferase 1, LCB1, LCB 1, HSAN1, EC:2.3.1.50

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Validation Data Gallery

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  WB analysis using 66899-1-Ig (same clone as 66899-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66899-1-Ig (SPTLC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66899-1-Ig (same clone as 66899-1-PBS)

  WB result of SPTLC1 antibody (66899-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPTLC1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66899-1-Ig (same clone as 66899-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66899-1-Ig (SPTLC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66899-1-Ig (same clone as 66899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66899-1-Ig (SPTLC1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66899-1-Ig (same clone as 66899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66899-1-Ig (SPTLC1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50865-2

  Cytometric bead array standard curve of MP50865-2, SPTLC1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66899-4-PBS. Detection antibody: 66899-1-PBS. Standard:Ag7876. Range: 0.195-100 ng/mL.

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Product Information

66899-1-PBS targets SPTLC1 as part of a matched antibody pair:

MP50865-2: 66899-4-PBS capture and 66899-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SPTLC1 fusion protein Ag7876
• Full Name: serine palmitoyltransferase, long chain base subunit 1
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC007085
• Gene Symbol: SPTLC1
• Gene ID (NCBI): 10558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SPTLC1 is a subunit of serine palmitoyltransferase (SPT) which is the key enzyme in sphingolipid biosynthesis and is essential for embryogenesis and cell survival. Mutations in the SPTLC1 gene (C133W, C133Y, V144D, and G387A) were reported to be responsible for the development of an inherited sensory neuropathy (hereditary sensory neuropathy type I, HSN1).