SPTLC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SPTLC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 15376-1-AP

Synonyms

HSAN, HSAN1, HSN1, LBC1, LCB 1, LCB1, Serine palmitoyltransferase 1, SPT 1, SPT1, SPTI, SPTLC1

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Validation Data Gallery

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  WB analysis of Jurkat using 15376-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15376-1-AP (SPTLC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15376-1-AP

  WB result of SPTLC1 antibody (15376-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPTLC1 transfected HEK-293 cells.

• 

  WB analysis of HepG2 using 15376-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15376-1-AP (SPTLC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15376-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15376-1-AP (SPTLC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15376-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15376-1-AP (SPTLC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15376-1-AP

  IP result of anti-SPTLC1 (IP:15376-1-AP, 4ug; Detection:15376-1-AP 1:500) with HEK-293 cells lysate 880ug.

• 

  IHC staining of human colon cancer using 15376-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15376-1-AP (SPTLC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 15376-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15376-1-AP (SPTLC1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, human liver tissue, HepG2 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 2 publications below

Product Information

15376-1-AP targets SPTLC1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPTLC1 fusion protein Ag1162
• Full Name: serine palmitoyltransferase, long chain base subunit 1
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC007085
• Gene Symbol: SPTLC1
• Gene ID (NCBI): 10558
• RRID: AB_2286678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPTLC1 is a subunit of serine palmitoyltransferase (SPT) which is the key enzyme in sphingolipid biosynthesis and is essential for embryogenesis and cell survival. Mutations in the SPTLC1 gene (C133W, C133Y, V144D, and G387A) were reported to be responsible for the development of an inherited sensory neuropathy (hereditary sensory neuropathy type I, HSN1).

Publications

Species	Application	Title
rat	IHC	Cell Metab; Central Ceramide Signaling Mediates Obesity-Induced Precocious Puberty.; Authors - Violeta Heras
human	WB	Sci Adv; Inhibition of sphingolipid de novo synthesis counteracts muscular dystrophy.; Authors - Pirkka-Pekka Laurila
mouse	WB	J Neurosci; MicroRNA-137/181c regulates serine palmitoyltransferase and in turn amyloid β, novel targets in sporadic Alzheimer's disease.; Authors - Hirosha Geekiyanage
mouse	WB,IHC	Neurobiol Aging; Inhibition of serine palmitoyltransferase reduces Aβ and tau hyperphosphorylation in a murine model: a safe therapeutic strategy for Alzheimer's disease.; Authors - Hirosha Geekiyanage
mouse	WB	Am J Physiol Lung Cell Mol Physiol; Neutrophil elastase increases airway ceramide levels via upregulation of serine palmitoyltransferase.; Authors - Sophia Karandashova
human	WB	Front Cell Neurosci; Activation of Mitochondrial Unfolded Protein Response in SHSY5Y Expressing APP Cells and APP/PS1 Mice.; Authors - Yang Shen ; KD Validated