SPTBN4 Polyclonal antibody

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  WB analysis using 31713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31713-1-AP (SPTBN4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 31713-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SPTBN4 antibody (31713-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

SPTBN4 Polyclonal Antibody for WB, ELISA

Cat No. 31713-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

SPTBN3, KIAA1642, Beta IV spectrin

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31713-1-AP targets SPTBN4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPTBN4 fusion protein Ag36430
• Full Name: spectrin, beta, non-erythrocytic 4
• Calculated Molecular Weight: 288kDa
• Observed Molecular Weight: 250, 140 kDa
• GenBank Accession Number: NM_020971
• Gene Symbol: SPTBN4
• Gene ID (NCBI): 57731
• RRID: AB_3670092
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9H254
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPTBN4 also known as beta-IV spectrin, encodes a nonerythrocytic member of the beta-spectrin protein family that is expressed in the brain, peripheral nervous system, pancreas, and skeletal muscle. Spectrins are rod-shaped proteins that were originally identified as part of the lattice-like cytoskeleton under the erythrocyte membrane(PMID: 11086001).