SPTBN2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SPTBN2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 55107-1-AP

Synonyms

Spinocerebellar ataxia 5 protein, Spectrin beta-3, Spectrin beta chain, non-erythrocytic 2, Beta-III spectrin, Beta III spectrin

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Validation Data Gallery

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  WB analysis using 55107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55107-1-AP (SPTBN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55107-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55107-1-AP (SPTBN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 55107-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55107-1-AP (SPTBN2 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 55107-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55107-1-AP (SPTBN2 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, mouse brain, rat brain
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Product Information

55107-1-AP targets SPTBN2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: spectrin, beta, non-erythrocytic 2
• Calculated Molecular Weight: 271 kDa
• Observed Molecular Weight: 270 kDa
• GenBank Accession Number: NM_006946
• Gene Symbol: SPTBN2
• Gene ID (NCBI): 6712
• RRID: AB_10804779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPTBN2, also named as KIAA0302, belongs to the spectrin family. It probably plays an important role in neuronal membrane skeleton. Defects in SPTBN2 are the cause of spinocerebellar ataxia type 5 (SCA5). The antibody is specific to SPTBN2.

Publications

Species	Application	Title
Drosophila	WB, IF	J Biol Chem; β-Spectrin regulates the Hippo signaling pathway and modulates the basal actin network.; Authors - Kenneth Kin Lam Wong ; KD Validated
mouse	WB	Front Mol Neurosci; Analyzing the mechanisms that facilitate the subtype-specific assembly of γ-aminobutyric acid type A receptors; Authors - Catherine Choi
mouse	WB	Cerebellum; Nitric Oxide Critically Regulates Purkinje Neuron Dendritic Development Through a Metabotropic Glutamate Receptor Type 1-Mediated Mechanism.; Authors - Vasiliki Tellios
human	WB	Dis Markers; SPTBN2 Promotes the Progression of Thyroid Cancer by Accelerating G1/S Transition and Inhibiting Apoptosis; Authors - Xiaofen Zhou
human	WB	Exp Ther Med; Decreased SPTBN2 expression regulated by the ceRNA network is associated with poor prognosis and immune infiltration in low‑grade glioma; Authors - Guo-Rong Chen