SPTBN1-Specific Polyclonal antibody

SPTBN1-Specific Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 19722-1-AP

Synonyms

SPTBN1, Spectrin, non-erythroid beta chain 1, Spectrin beta chain, non-erythrocytic 1, Beta-II spectrin, Beta II spectrin

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Validation Data Gallery

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  WB analysis of mouse brain using 19722-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 19722-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 19722-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 19722-1-AP

  IP result of anti-SPTBN1-Specific (IP:19722-1-AP, 5ug; Detection:19722-1-AP 1:600) with HEK-293 cells lysate 1000ug.

• 

  IHC staining of human liver cancer using 19722-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 19722-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 19722-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 19722-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 19722-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 19722-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19722-1-AP (SPTBN1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse kidney tissue, mouse lung tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver cancer tissue, human lung tissue, human kidney tissue, human pancreas cancer tissue, human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

19722-1-AP targets SPTBN1-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: spectrin, beta, non-erythrocytic 1
• Calculated Molecular Weight: 275 kDa
• Observed Molecular Weight: 275 kDa
• GenBank Accession Number: NM_003128
• Gene Symbol: SPTBN1
• Gene ID (NCBI): 6711
• RRID: AB_10638469
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPTBN1 is the non-erythrocytic form of β-spectrin or β-fodrin. Spectrins are members of the superfamily of F-actin cross linking proteins that are important as scaffolding proteins for protein sorting, cell adhesion, and migration. Reduced expression of SPTBN1 has been found to correlate with shorter survival of patients with hepatocellular cancer, pancreatic cancer and other malignancies of the gastrointestinal tract, suggesting tumor suppression ability of this protein.

Publications

Species	Application	Title
human	WB,IP	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
human	WB,IHC	Proteomics; Global profiling of proteolytically modified proteins in human metastatic hepatocellular carcinoma cell lines reveals CAPN2 centered network.; Authors - Shen Chengpin C
human	IF	Cancer Med; MiR-362 suppresses cervical cancer progression via directly targeting BAP31 and activating TGFβ/Smad pathway.; Authors - Shuya Yang
mouse	IF	FEBS Open Bio; Identification of nuclear phosphoproteins as novel tobacco markers in mouse lung tissue following short-term exposure to tobacco smoke.; Authors - Kanako Niimori-Kita
mouse,human	WB	Neuron; Species-specific FMRP regulation of RACK1 is critical for prenatal cortical development; Authors - Minjie Shen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ruchi (Verified Customer) (01-25-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse skin