SPTBN1 Polyclonal antibody

SPTBN1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25681-1-AP

Synonyms

Beta II spectrin, betaSpII, ELF, Fodrin beta chain, Spectrin beta chain, brain 1, SPTB2, SPTBN1

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Validation Data Gallery

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  WB analysis of HEK-293 using 25681-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25681-1-AP (SPTBN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 25681-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25681-1-AP (SPTBN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 25681-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25681-1-AP (SPTBN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 25681-1-AP

  Immunofluorescent analysis of HEK-293 cells using 25681-1-AP (SPTBN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Caco-2 cells, mouse heart tissue, HEK-293 cells, SH-SY5Y cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25681-1-AP targets SPTBN1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPTBN1 fusion protein Ag22459
• Full Name: spectrin, beta, non-erythrocytic 1
• Calculated Molecular Weight: 2364 aa, 275 kDa
• Observed Molecular Weight: 275 kDa
• GenBank Accession Number: BC137282
• Gene Symbol: SPTBN1
• Gene ID (NCBI): 6711
• RRID: AB_2880191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPTBN1 is the non-erythrocytic form of β-spectrin or β-fodrin. Spectrins are members of the superfamily of F-actin cross linking proteins that are important as scaffolding proteins for protein sorting, cell adhesion, and migration. Reduced expression of SPTBN1 has been found to correlate with shorter survival of patients with hepatocellular cancer, pancreatic cancer and other malignancies of the gastrointestinal tract, suggesting tumor suppression ability of this protein.