Spartan Polyclonal antibody

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  WB analysis of human placenta using 24850-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 24850-1-AP (Spartan antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24850-1-AP

  IP result of anti-Spartan (IP:24850-1-AP, 4ug; Detection:24850-1-AP 1:500) with HEK-293 cells lysate 1800 ug.

Spartan Polyclonal Antibody for WB, IP, ELISA

Cat No. 24850-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

C1orf124, DVC1, SPRTN, PRO4323, DDDL1880

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24850-1-AP targets Spartan in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Spartan fusion protein Ag20586
• Full Name: DNA-dependent metalloprotease SPRTN
• Calculated Molecular Weight: 489 aa, 55 kDa
• Observed Molecular Weight: 55 kDa, 65 kDa
• GenBank Accession Number: BC015740
• Gene Symbol: Spartan
• Gene ID (NCBI): 83932
• RRID: AB_3669456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H040
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Spartan (Protein with SprT-like domain at the N terminus), also known as DVC1 or C1orf124, is a replication-coupled DNA-dependent metalloprotease that cleaves proteins cross-linked to DNA to promote DPC repair (PMID: 38600235). Spartan protease activity is stimulated by DNA binding, while posttranslational modification of Spartan governs both its protease activity and recruitment to the DPC on chromatin (PMID: 27871366). The regulation of Spartan includes a ubiquitin switch (where deubiquitination activates its proteolytic activity), and acetylation and phosphorylation switches (which facilitate DNA binding). The calculated molecular weight of Spartan is 55 kDa, while the 65-kDa band could be due to posttranslational modification (PMID: 33567341, 31316063).