SPRR3 antibody (11742-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 11742-1-AP (same clone as 11742-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 11742-1-AP (SPRR3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.
WB analysis of human liver using 11742-1-AP (same clone as 11742-1-PBS)
human liver tissue were subjected to SDS PAGE followed by western blot with 11742-1-AP (SPRR3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.
IP experiment of COLO 320 using 11742-1-AP (same clone as 11742-1-PBS)
IP result of anti-SPRR3 (IP:11742-1-AP, 3ug; Detection:11742-1-AP 1:500) with COLO 320 cells lysate 1720ug. This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 11742-1-AP (same clone as 11742-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 11742-1-AP (same clone as 11742-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 11742-1-AP (same clone as 11742-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 11742-1-AP (same clone as 11742-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 11742-1-AP (same clone as 11742-1-PBS)
Immunofluorescent analysis of HeLa cells using 11742-1-AP (SPRR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11742-1-PBS in a different storage buffer formulation.

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Tested Applications
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Product Information

11742-1-PBS targets SPRR3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SPRR3 fusion protein Ag2337 Predict reactive species
Full Name	small proline-rich protein 3
Calculated Molecular Weight	19 kDa
Observed Molecular Weight	22-25 kDa
GenBank Accession Number	BC017802
Gene Symbol	SPRR3
Gene ID (NCBI)	6707
RRID	AB_2195272
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UBC9
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SPRR3, also named as Esophagin, Cornifin beta and SPRC, belongs to the cornifin (SPRR) family. It is cross-linked envelope protein of keratinocytes. Increased expression of SPRR3 appears to be involved in colorectal tumorigenesis. SPRR3 is a marker for terminal squamous cell differentiation.a potential novel therapeutic target for less advanced stages of Breast Cancer.