SPRR1B Polyclonal antibody

SPRR1B Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11959-1-AP

Synonyms

14.9 kDa pancornulin, CORNIFIN, Cornifin B, GADD33, Small proline rich protein IB, SPR IB, SPRR1, SPRR1B

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Validation Data Gallery

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  IHC staining of human skin using 11959-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11959-1-AP (SPRR1B Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 11959-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11959-1-AP (SPRR1B Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 11959-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11959-1-AP (SPRR1B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 11959-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11959-1-AP (SPRR1B Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

11959-1-AP targets SPRR1B in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPRR1B fusion protein Ag2563
• Full Name: small proline-rich protein 1B (cornifin)
• Calculated Molecular Weight: 89 aa, 10 kDa
• GenBank Accession Number: BC056240
• Gene Symbol: SPRR1B
• Gene ID (NCBI): 6699
• RRID: AB_2877807
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This protein functions as a component of the cross-linked envelope in squamous differentiating cells. It runs as a 15-kDa protein in unreducing SDS PAGE, whereas under reducing conditions it runs as 23 kDa one.

Publications

Species	Application	Title
human	IHC	Cell Discov; Single-cell atlas of keratoconus corneas revealed aberrant transcriptional signatures and implicated mechanical stretch as a trigger for keratoconus pathogenesis.; Authors - Shengqian Dou
human	IHC	Front Oncol; Long Noncoding RNA HAGLROS Promotes the Malignant Progression of Bladder Cancer by Regulating the miR-330-5p/SPRR1B Axis.; Authors - Shiwei Xiao