SPR Polyclonal antibody, PBS Only

SPR Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16822-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 16822-1-AP (same clone as 16822-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16822-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 16822-1-AP (same clone as 16822-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16822-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 16822-1-AP (same clone as 16822-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16822-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 16822-1-AP (same clone as 16822-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16822-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16822-1-AP (same clone as 16822-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16822-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 16822-1-AP (same clone as 16822-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16822-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse liver using 16822-1-AP (same clone as 16822-1-PBS)

  IP result of anti-SPR (IP:16822-1-AP, 4ug; Detection:16822-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 16822-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 16822-1-AP (same clone as 16822-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16822-1-AP (SPR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16822-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 16822-1-AP (same clone as 16822-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16822-1-AP (SPR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16822-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 16822-1-AP (same clone as 16822-1-PBS)

  Immunofluorescent analysis of Hela cells, using SPR antibody 16822-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16822-1-PBS in a different storage buffer formulation.

---

Product Information

16822-1-PBS targets SPR in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPR fusion protein Ag10395
• Full Name: sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase)
• Calculated Molecular Weight: 261 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC017310
• Gene Symbol: SPR
• Gene ID (NCBI): 6697
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.