SPPL2A Polyclonal antibody

SPPL2A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13255-1-AP

Synonyms

IMP 3, IMP3, Intramembrane protease 3, Presenilin like protein 2, PSL2, SPP like 2A, SPPL2A

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Validation Data Gallery

• 

  WB analysis of human colon using 13255-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13255-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 13255-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13255-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 13255-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human colon tissue, Jurkat cells, mouse colon tissue
• Positive IHC detected in: human testis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13255-1-AP targets SPPL2A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SPPL2A fusion protein Ag3935
• Full Name: signal peptide peptidase-like 2A
• Calculated Molecular Weight: 520 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC025740
• Gene Symbol: SPPL2A
• Gene ID (NCBI): 84888
• RRID: AB_2195000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPPL2A(Signal peptide peptidase-like 2A) is also named as IMP3(Intramembrane protease 3), PSL2(Presenilin-like protein 2) and belongs to the peptidase A22B family. It is an aspartyl intramembrane protease, has been implicated in the proteolysis of TNF-alpha, Fas Ligand and Bri2 and endogenous signal-peptide-peptidase-like2A (SPPL2a) is localised in lysosomes/late endosomes(PMID:21896273). This protein has a signal peptide with 25 amino acid and seven glycosylation sites.